Co-cultures | Control | 100 μm CBX | Wash | Control | 100 μm GZA |
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Baseline (F0) (a.u.) | 899 ± 45 | 971 ± 46 | 921 ± 76 | 870 ± 32 | 848 ± 36 |
Peak normalised amplitude (ΔF/F0) | 190 ± 20 | 180 ± 20 | 160 ± 10 | 220 ± 10 | 230 ± 10 |
Rise time (s) | 1.48 ± 0.16 | 1.57 ± 0.11 | 1.38 ± 0.17 | 0.98 ± 0.12 | 1.08 ± 0.13 |
Half-decay time (s) | 6.48 ± 0.25 | 9.69 ± 1.18** | 7.55 ± 0.68 | 6.38 ± 0.22 | 6.58 ± 0.20 |
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Enriched neuronal cultures | Control | 100 μm CBX | Wash | ||
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Baseline (F0) (a.u.) | 810 ± 28 | 808 ± 32 | 802 ± 28 | ||
Peak normalised amplitude (ΔF/F0) | 200 ± 10 | 190 ± 10 | 180 ± 10 | ||
Rise time (s) | 0.79 ± 0.05 | 0.80 ± 0.05 | 0.76 ± 0.07 | ||
Half-decay time (s) | 7.25 ± 0.58 | 6.38 ± 0.46 | 6.03 ± 0.61 |
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Data are expressed as mean ±s.e.m. and were obtained from 4 and 5 independent experiments in co-cultures and enriched neuronal cultures, respectively. Neurons were recorded before (control), after perfusion with carbenoxolone (CBX, 100 μm, 10 min) and wash or after glycyrrhizic acid (GZA, 100 μm, 10 min). ANOVA followed by post hoc Bonferroni's multiple comparison (CBX) or Student's unpaired t test (GZA) were applied for statistical analysis
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↵** P < 0.01.